大鼠脂联素检测试剂盒（酶联免疫法）产品说明:

【产品名称】

通用名称：大鼠脂联素检测试剂盒（酶联免疫法）

英文名称：Rat-Adiponectin ELISA

【预期用途】

用于定大鼠血清和血浆中脂联素。仅供科研使用。不用于临床诊断。

【包装规格】

96T/盒

【背景知识】

脂联素是一种30 kDa的蛋白质，在血清中以高浓度（0.01%）存在，该蛋白质主要由脂肪细胞合成，但肌肉细胞和肝细胞也能合成脂联素。它由一个胶原样 N 端和一个球状 C 端结构域组成 [1]。在体内，脂联素存在于不同的寡聚体中。除三聚体和二三聚体外，还存在高分子多聚体[1-3]。已知有两种不同的受体，两种受体都普遍表达，但在组织中的分布不同。脂联素受体 1 (AdipoR1) 特别在肌肉中合成，而 AdipoR2 特别在肝组织中合成 [4]。

研究表明，脂联素与 BMI 呈负相关，因此可能对能量代谢很重要，例如通过调节脂肪酸氧化与 ll 型糖尿病的联系。脂联素也代表了葡萄糖和脂肪代谢之间的联系 [8, 9]。

不同描述的高分子多聚体的特殊诊断价值无法在三种商业测试系统的比较研究中得到证明 [10]。使用 ROC 分析，Blüher 等人能够确定诊断胰岛素抵抗的“曲线下面积”为 0.92。这表明使用 Mediagnost E09 [10] 测量脂联素可能具有诊断益处。

此外，脂联素参与炎症过程 [11-15]，因此对于动脉硬化 [4, 5, 16] 和冠状动脉炎症 [17, 18] 的发展很重要，以估计冠状动脉疾病 [19,20]。脂联素还影响其他生理过程，例如血管生成 [21,22]。

参考文献：

1. Nakano, Y. et al, Isolation and characterization of GBP28,a novel gelatin-binding protein purified fromhuman plasma.J Biochem (Tokyo), 1996.120(4): p.803-12.

2. Pajvani, U.B.,et al，Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin.lmplications fpr metabolic regulation and bioactivity.J Biol Chem, 2003.278(11): p.9073-85.

3. Tsao,T.S. et al., Role of disulfide bonds in Acrp30ladiponectin structure and signaling specificity. Differentoligomers activate different signal transduction pathways.J Biol Chem, 2003.278(50): p. 50810-7.

4. Shimada，K., T.Mliyazaki, and H. Daida,Adiponectin and atherosclerotic disease.Clin Chim Acta, 2004.344(1-2): p.1-12.

5. Higashiura，K., et al.，Correlations of adiponectin level with insulin resistance and atherosclerosis inJapanese male populations. Clin Endocrinol (Oxf), 2004.61(6): p.753-9.

6. Spranger，J., et al，Adiponectin is independently associated with insulin sensitivity in women withpolycystic ovary syndrome.Clin Endocrinol (Oxf), 2004.61(6): p.738-46.

7. Zoico，E.,et al.,Adipocytokines,fat distribution，and insulin resistance in elderly men and women. JGerontol A Biol Sci Med Sci,2004.59(9): p.M935-9.

8. Ye, J.M., et al, PPARalpha gamma ragaglitazar eliminates fatty liver and enhances insulin action in fat-fed rats in the absence of hepatomegaly.Am J Physiol Endocrinol metab,2003.284(3): p.E531-40.

9. Yamauchi, T. et al.,Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nat Med,2002.8(11): p. 1288-95.

10. Blüher(Blueher), M., et al.,Total and high-molecular weight adiponectin in relation to metabolic variablesat baseline and in response to an exercise treatment program: comparative evaluation of three assays.Diabetes Care, 2007.30(2): p.280-5.

11. Delaigle,A.M., et al., lnduction of adiponectin in skeletal muscle by inflammatory cytokines: in vivo and in vitro studies.Endocrinology, 2004.145(12): p.5589-97.

12. Winzer,C. et al., Plasma adiponectin, insulin sensitivity, and subclinical inflammation in women with prior gestational diabetes mellitus. Diabetes Care, 2004.27(7): p.1721-7.

13. Xydakis,A.M., et al, Adiponectin, inflammation, and the expression of the metabolic syndrome in obese individuals: the impact of rapid weight loss through caloric restriction.J Clin Endocrinol metab, 2004.89(6):p.2697-703.

14. Motoshima,H, et al.Adiponectin suppresses proliferation and superoxide generation and enhances eNOS activity in endothelial cells treated with oxidized LDL.Biochem Biophys Res Commun,2004.315(2):p.264-71.

15. wWolf，A.M.， et al，Adiponectin induces the anti-inflammatory cytokines lL-10 and l-1RA in humanleukocytes. Biochem Biophys Res Commun,2004.323(2): p.630-5.

16. Okamoto,Y., et al.,Adiponectin reduces atherosclerosis in apolipoprotein E-deficient mice. Circulation,2002.106(22): p.2767-70.

17. Schlegel,A., Adiponectin and risk of coronary heart disease.Jama,2004.292(1): p. 40; author reply 40.18. Choi,K.M. et al., Inflammation,Insulin Resistance, and Glucose Intolerance in Acute Myocardial Infarction

18. Patients without a Previous Diagnosis of Diabetes Mellitus.J Clin Endocrinol metab, 2004.

19. Nakamura,Y., et al., lrmplications of plasma concentrations of adiponectin in patients with coronary artery disease. Heart,2004.90(5): p.528-33.

20. Pischon，T., et al.，Plasma adiponectin levels and risk of myocardial infarction in men. Jama, 2004.291 (14): p.1730-7.

21. Shibata, R., et al., Adiponectin stimulates angiogenesis in response to tissue ischemia through stimulation of amp-activated protein kinase signaling.J Biol Chem, 2004.279(27): p.28670-4.

22. Fernandez-Real,J.M., et al.，Adiponectin is associated with vascular function independent of insulin sensitivity. Diabetes Care, 2004.27(3): p.739-45.

招商政策:

①及时提供货源，确保全国范围内2～10天内到货；
②实行区域代理，确保运作者权益；
③提供合理的运作空间，确保投入与收益成正比；
④企业会持续稳定给予代理商做全面服务工作；
⑤完成任务年终返点；
⑥提供合法的经营手续；

代理商要求:

①有长期合作的决心；
②具有较强的责任心和信心；
③完善的销售网络；
④具有一定的经济实力和良好的商业信誉；
⑤具有丰富的市场操作经验，较强的市场开发能力；