阿狄科®总可溶性破骨细胞异化因子检测试剂盒（酶联免疫法）产品说明:

【产品名称】

通用名称：阿狄科®总可溶性破骨细胞异化因子检测试剂盒（酶联免疫法）

英文名称：total/sRANKL

【包装规格】

96人份/盒

【预期用途】

用于血清和血浆中总 sRANKL(human) 的定量测定。仅供科研使用。

【背景知识】

RANKL（NF-kB 配体的受体激活剂，也称为骨保护素配体，OPGL），其细胞受体 RANK 及其清道夫受体骨保护素 (OPG) 已被确定为人体内在骨重塑电路中的关键成分。 RANKL 是 TNF（肿瘤坏死因子）家族的成员，是破骨细胞成熟的主要刺激因素，对它们的生存至关重要。因此，RANKL 表达的增加导致骨吸收和丢失。RANKL 由成骨细胞谱系和活化的 T 淋巴细胞产生，并激活其位于破骨细胞和树突状细胞上的特异性受体 RANK。

RANKL 的作用受 OPG 控制，OPG 分泌于各种组织中，作为可溶性内源性受体拮抗剂。

佩吉特病、良性和恶性骨肿瘤、绝经后骨质疏松症、类风湿性关节炎、骨转移和高钙血症的发病机制与 RANKL/OPG 的不平衡有关，可以通过添加 OPG 来调节 RANKL/OPG 平衡。

RANKL 已显示在鼠成骨细胞或基质细胞中作为膜结合蛋白表达，并被金属蛋白酶切割成可溶形式 (sRANKL)。TNFa 还促进 RANKL 的表达并抑制 OPG 在成骨细胞或基质细胞中的表达细胞因子如 IL-13、INF-g 和 TNF-α1，它们抑制破骨细胞生成，抑制 RANKL 的表达并刺激 OPG 的表达。

适应症：

绝经后和老年性骨质疏松症

局部骨吸收增加的疾病

佩吉特病

牙周病

炎症性疾病

免疫系统疾病

关节炎

肿瘤学

参考文献：

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2. Kong Y.Y.et al., OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis.Nature (1999),397:315-323.

3. Hsu H.et al.,Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand. Proc NatlAcad Sci (1999),96:3540-3545.

4. Josien R, et al, TRANCE, a tumor necrosis factor family member, enhances the longevity and adjuvant properties of dendritic cells in vivo.JExp Med (2000),191:495-502.

5. Fuller K.et al.,TRANCEis necessary and sufficient for osteoblast-mediated activation of bone resorption in osteoclasts. JExp Med (1998),188:997-1001.

6. Nakashima T , et. al., Protein expression and functional difference of membrane bound and soluble receptor activator of NF-kappaB ligand: modulation of theexpression by osteotropic factors and cytokines. Biochem Biophys Res Commun(2000), 275(3):768-75.

7. Kong Y.Y.et al,Activated T cells regulate bone loss and joint destruction in adjuvant arthritis through osteoprotegerin ligand.Nature (1999),402:304-309.

8. Hofbauer L.C.&A.E.Heufelder, Role of receptor activator of nuclear factor-KB ligand and osteoprotegerin in bone cell biology.J Mol Med (2001),79:243-253.

9. Hofbauer L.C.& A.E.Heufelder, The Role of Osteoprotegerin and Receptor Activator of Nuclear Factor KB Ligand in the Pathogenesis and Treatment of Rheumato-id Arthritis.Arthritis & Rheumatism (2001),44:253-259.

10.Hofbauer LC, et al...The role of receptor activator of nuclear factor-kappaB ligand and osteoprotegerin in the pathogenesis and treatment of metabolic bone di-seases.JCin Endocrinol metab (2000),85: 2355-2363.

11.Teitelbaum S.L., Bone resorption by osteoclasts.Science (2000), 289:1504-1508.

12.Boumans,M.J.H. et al., 2012.Rituximab abrogates joint destruction in rheumatoid arthritis by inhibiting osteoclastogenesis.Annals of the rheumatic diseases,71(1),pp.108-13.

13. Dovio, A.et al., 2008.Circulating osteoprotegerin and soluble RANK ligand in systemic sclerosis.The Journal of rheumatology, 35(11), pp.2206-13.

14.Dovio, A.et al., 2007.Increased osteoprotegerin levels in Cushings syndrome are associated with an adverse cardiovascular risk profile.The Journal of clinical endo-crinology and metabolism,92(5),pp.1803-8.

15.Findlay, D. et al., 2008.Circulating RANKL is inversely related to RANKL mRNA levels in bone in osteoarthritic males.Arthritis research & therapy,10(1), p.R2.

16.Gonzélez-Alvaro, l.et al., 2007.baseline serum RANKL levels may serve to predict remission in rheumatoid arthritis patients treated with TNF antagonists.Annals ofthe rheumatic diseases, 66(12), pp.1675-8.

17.Hein, G. et al., 2000. vergleich der Serum- und Synovia-Spiegel von sRANKL und OPG bei rheumatoider Arthritis und nicht erosiven Arthritiden.Poster beim Kon-gress der Deutschen Gesellschaft fur Rheumatologie. Dresden.

18.Hein, G.E. et al.,2008. sRANKL and OPG in serum and synovial fluid of patients with rheumatoid arthritis in comparison to non-destructive chronic arthritis.Rheumatology international, 28(8), pp.765-9.

19.Hofbauer, L.C. et al., 2004.Effects of oral contraceptives on circulating osteoprotegerin and soluble RANK ligand serum levels in healthy young women.Clinicalendocrinology, 60(2).pp.214-9.

20.Kamiya,N. et al, 2011. Significance of serum osteoprotegerin and receptor activator of nuclear factor xB ligand in Japanese prostate cancer patients with bonemetastasis. lnternational journal of clinical oncology,16(4), pp.366-72.

21.Kerschan-Schindl, K. et al., 2008. Serum levels of receptor activator of nuclear fac-

tor kappaB ligand (RANKL) in healthy women and men.Experimental and clinicalendocrinology & diabetes : official journal, German Society of Endocrinology [and]German Diabetes Association, 116(8).pp.491-5.

22.Li, E.K. et al., 2009.High prevalence of asymptomatic vertebral fractures in Chinese women with systemic lupus erythematosus.The Journal of rheumatology,36(8), pp.1646-52.

23.Nielen, M. et al.，, 2004.Markers of bone formation and resorption in preclinical rheumatoid arthritis are associated with radiographic progression. Poster beimAmerican College of Rheumatology Meeting. San Diego.

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25.Oelzner, P. et al., 2006.RANKL ，Osteoprotegerin und IlL-6-System bei Rheumatoider Arthritis -EinfluR von Alter ，Erkrankungsdauer , Menopause und entzund-licher Aktivitat.Poster bei Osteologie.Koln.

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