阿狄科®羰基蛋白质检测试剂盒（酶联免疫法）（生物样本）产品说明:

【产品名称】

通用名称：阿狄科®羰基蛋白质检测试剂盒（酶联免疫法）（生物样本）

英文名称：Carbonylated Proteins

【包装规格】

96人份/盒

【预期用途】

用于测定生物样品中的蛋白质结合羰基化合物，例如 EDTA 血浆、支气管肺泡灌洗液和脑脊液、细胞提取物和其他可溶性蛋白质样品。仅供科研使用。

【背景知识】

活性氧 (ROS) 可以氧化细胞中的蛋白质、脂质和 DNA，从而在结构和功能上破坏它们。蛋白质被自由基氧化，以各种方式修饰或降解其氨基酸。蛋白质接受新的官能团，如羰基或羟基，可导致蛋白质断裂、交联形成、三级结构破坏和功能丧失。活性氧还与动脉粥样硬化、阿尔茨海默氏症、帕金森氏症和类风湿性关节炎等疾病直接相关以及衰老过程和致癌作用。

蛋白质结合羰基化合物由多种氧化机制形成，是氧化损伤的敏感标志物。蛋白质结合羰基化合物的含量可以通过用DNPH衍生化并随后测定结合的抗 DNPH 抗体来确定。 ELISA 方法能够以微克范围内的蛋白质含量进行定量羰基测定。

适应症：

动脉硬化

阿尔茨海默氏症

帕金森氏症

类风湿关节炎

尿毒症

糖尿病

衰老过程

致癌

 参考文献：

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4. Cao, G.& Cutler,R.G.Protein oxidation and aging.I. Difficulties in measuring re-active protein carbonyls in tissues using 2,4-dinitrophenylhydrazine.Archives ofbiochemistry and biophysics 320,106-14(1995).

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9. Gladstone,L. M.& Levine, R.L. Oxidation of proteins in neonatal lungs. Pediatrics93,764-8 (1994).

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